Book traversal links for Autoclave Cycles ‹ Autoclave Use Up Compatible/Incompatible Materials › To be effective, the autoclave must reach and maintain a temperature of 121° C for at least 30 minutes by using saturated steam under at least 15 psi of pressure. Increased cycle time may be necessary depending upon the make-up and volume of the load. The rate of exhaust will depend upon the nature of the load. Dry material can be treated in a fast exhaust cycle, while liquids and biological waste require slow exhaust to prevent boiling over of super-heated liquids. Material Recommended for: Liquids cycle (Slow Exhaust) Use with glass containers with vented closures; 2/3 full only •Liquid media •Nonflammable liquids •Aqueous solutions •Liquid biological waste Solids or Dry cycle (Fast Exhaust) Glassware: empty and inverted no tight or impermeable closures Dry hard items, either unwrapped or in porous wrap Metal items with porous parts Other porous materials Wrapped Goods or Pre vacuum cycle (Clean: Fast Exhaust Dirty: Slow Exhaust) Glassware that must be sterilized upright and/or can trap air Wrapped dry items that can trap air Pipette tip boxes Sharps decontamination (in collection containers) Biohazard waste decontamination, in autoclave bags; can be wet or dry Book traversal links for Autoclave Cycles ‹ Autoclave Use Up Compatible/Incompatible Materials › Staff Meagan Fitzpatrick Biosafety Officer 609-258-6258 Halina Staniszewska, Sr. Lab Safety Specialist
